Table 1.

Summary of single LPS administration protocols and results.

Mouse Strain and Treatment Protocol	Results and Methods Applied	Refs.
BALB/c, young adult* or old*; LPS-0127:B8; i.p.0.33mg/kg. Sacrificed at 4h	Elevated IL-1β and IL-6 mRNA (RT-PCR) and protein (ELISA)	[50]
Same protocol; Sacrificed at 24 h	Elevated IL-1β mRNA and IL-6 protein	[50]
BALB/c, old; same protocol; Sacrificed at 24 h	Elevated IL-1β and IL-6 mRNA and IL-6 protein	[50]
C57BL/6J, ME7-infected (prion-disease model); i.p. 0.5mg/kg. Sacrificed at 6 h	Elevated IL-1β, IL-6 and TNF-α mRNA (RT-PCR) and IL-1β protein (ICH)	[37]
C57BL/6J; LPS-0111:B4; i.p.5mg/kg. Sacrificed at 1 h, 14 and 21 days, 10 months	Elevated TNF-α mRNA and protein (ELISA)	[22]
BALB/c, young adult and old; LPS-0127:B8; i.p.0.33mg/kg. Sacrificed at 4 h	Elevated IL-1β, IL-6 and TNF-α mRNA (RT-PCR)	[51]
BALB/c, young adult and old; LPS-0127:B8; i.p. 0.33mg/kg. Sacrificed at 4 h or 8 h	Elevated IL-1β mRNA (RT-PCR) and protein (FACS)	[52]
C57BL/6J, young adult; LPS-026:B6; i.p.2mg/kg. sacrificed at 4 h or 24 h	No effect observed	[53]
C57BL/6J, young adult; LPS-Salmonella Typhimurium; i.p.1mg/kg. Sacrificed at 4 days	Elevated IL-1β protein (ELISA)	[35]
ICR, young; LPS-Salmonella Typhimurium; i.p.5mg/kg. Sacrificed at 24 h	Elevated IL-6 protein (WB)	[54]
C57BL/6J, young; LPS-0111:B4; i.p. 2mg/kg. Sacrificed at 24 h	Elevated IL-1β, IL-6 and TNF-α mRNA and protein	[55]
B6C3F1, young adult; LPS-0111:B4; i.p. 10mg/kg. Sacrificed at 3 h	No statistically significant production of IL-1β, IL-6 and TNF-α protein (ELISA)	[56]
Same protocol. Sacrificed at 4,6 and 12 h	Elevated IL-6 protein (ELISA)	[56]

*young mice: 2 months of age; young adult mice: 3-4 months of age; adult mice: 12-13 months of age; old or aged mice: 20 or more months old.