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. 2016 Apr 8;12(4):e1005981. doi: 10.1371/journal.pgen.1005981

Fig 9. The DCRE recruits a Hox TF collective.

Fig 9

(A) Sequences of DCRE, RhoA, and DCRE-RhoA with Hox, Hth, Exd, FoxG, En, and Pax2 binding sites highlighted. Note that DCRE-RhoA lacks the En binding site required for posterior compartment repression. (B-C) GD-lacZ wild-type (B) and GD-RhoA-lacZ (C) activity visualized in Stage 15 embryos immunostained with β-gal (green or white) and Slp (magenta) demonstrates that the DCRE-RhoA element is capable of substantial abdominal repression. (D) Quantification of β-gal intensity relative to T3 segment shows that the DCRE-RhoA element is capable of substantial repression in the abdomen relative to the thorax, though it does not repress to DCRE wild-type levels. (** = p<0.01, Welch’s t-test). (E-F) Visualization of DMX-lacZ wild-type (E) and DMX-RhoA-lacZ (F) activity in stage 11 embryos immunostained for β-gal (red) and En (cyan) reveals that the DCRE-RhoA element represses in En negative cells of the abdomen. Note, that the thoracic levels activated by DCRE-RhoA-lacZ are decreased relative to DMX-lacZ wild-type. (G) Quantification of β-gal intensity in the thorax, the anterior abdominal compartment, and the posterior abdominal compartment of DMX-lacZ and DMX-RhoA-lacZ embryos. (* p<0.01, Tukey’s Test).