Table 1. The sequences of the primers used for PCR and the corresponding product sizes.
genes | primers(5’ - 3’) | products (bp) |
---|---|---|
SP1 | forward: AAGGATGCGGCAAAGTAT | 123 |
reverse: CGTCCGAACGTGTAAAGC | ||
ATF-2 | forward: GGAAAGTGTGGGTTCAGTCC | 150 |
reverse: GGAAAGTGTGGGTTCAGTCC | ||
SP3 | forward: GTGCTCGCATCTGTGGAA | 138 |
reverse: GTCTTGATTGCTGGTGGC | ||
C-MYB | forward: GCAGGCATTACCAACACAGA | 114 |
reverse: TCTCCCAAACAGGAAACAGG | ||
ICBP90 | forward: TCCAGTGCCGTTAAGACCTC | 102 |
reverse: ACGGACATTCTTGGCTTTGA | ||
Topo IIα | forward: GAGGCTTCCAGCAGATTAGC | 107 |
reverse: CACCACATCAACGAGTTTGC | ||
GAPDH | forward: GGGCATCTTGGGCTACACT | 209 |
reverse: GGTCCAGGGTTTCTTACTCC |