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. 2016 Mar 14;28(3):786–803. doi: 10.1105/tpc.15.00608

Figure 4.

Figure 4.

Expression Pattern and Genetic Regulation of PH3.

(A) Real-time RT-PCR analysis of PH3 mRNA in organs from stage 5-7 flowers (stage 5, fully elongated bud still closed; stage 6, almost fully open flower; stage 7, fully open flower with dehiscent anthers) of the wild-type line R27 and seeds from R27 and the isogenic an1 line W225. GAPDH mRNA served as a constitutive control.

(B) In situ hybridization of PH3, DFR, and AN1 in petals. The negative control was the DFR sense probe.

(C) Real-time RT-PCR of DFR, PH3, and PH5 mRNAs at different flower developmental stages in petals of the wild-type line R27 (red bar) and isogenic lines with an1, an11 (white bars), ph4, or ph2 mutations (purplish bars).

(D) Real-time RT-PCR analysis of PH3 mRNA in the petal limbs of detached stage 4 flowers of an an1 mutant (white bars) and an1 p35S:AN1-GR transgenic line (hatched bars) that were treated for 0, 2, or 24 h with DEX and/or cycloheximide (CHX).