Table 1. Description of the studied cytokines, polymorphisms (SNP and rs) restriction enzymes, primers sequences, annealing temperatures and the expected RFLP patterns.
| Cytokine RFLP enzyme Reference | SNP rs | PRIMER SEQUENCE Annealing temperature | RFLP pattern wild type homozygous heterozygous |
|---|---|---|---|
| IL6 NlaIII 15 | -174 G/C (rs1800795) | F: TGACTTCAGCTTTACTCTTGT R: CTGATTGGACTTATTAAG 51 °C | GG 167 e 31bp CC 122, 45, 31bp GC 167, 122, 45, 31bp |
| IL12p40 TaqaI 16 | +1188 A/C (rs3212227) | F:CTGATCCAGGATGAAAATTTGG R:CCCATGGCAACTTGAGAGCTGG 58 °C | AA 226 bp CC 185 e 41bp AC 226, 185,41bp |
| IL4 MnlI 17 | +33 C/T, (rs2070874) | F:CTCATTTTCCCTCGGTTTCAGC R:GAAGCAGTTGGGAGGTGAGA 60 °C | TT 126bp CC 82 e 44bp TC 126, 82 e 44bp |
| IL10 ApoI 18 | -3575 T/A (rs1800890) | F: GGTTTTCCTTCATTTGCAGC R: ACACTGTGAGCTTCTTGAGG 62 °C | TT 121 e 107 bp AA 228 bp TA 228, 121, 107 bp |
| TGFb1 NotI 19 | +869 T/C (rs1800470) | F:GTGCCGGGGGGCGCCGCCTCCCCCATGCCTCCCTC R:CAGCACCAGTAGCCACAGCAGCGGTAGCCGCAGC 60 °C | TT 199 bp CC 179 e 20 bp TC 199,179, 20 bp |
SNP - Single Nucleotide Polymorphism; F=forward; R= Reverse; bp= base pair; rs (SNP accession number)