Figure 4.

Effects of reduction on the cysteine mutant constructs. The strong reducing agent TCEP was used. This resulted in a small 40% inhibition of the WT channel. Constructs in α region II and γ regions I and II behaved as WT indicating no additional disulfides were formed by the introduced exogenous cysteines. Those in α region I were stimulated from WT and not different from no change indicating likely stabilization of the channel. N = 5–9. # indicates P < 0.05 when compared to no change. See Figure 3 legend for more details.