Figure 6.

Effects of thiol modification are altered in subtilisin cleaved channels. The effects of positive charge modification were examined in subtilisin cleaved channels. Modification of the WT channel was similar to that observed in Figure 5 for furin cleaved channels and exhibited an ∼40% inhibition. Modification to γ region II was not different from WT. However, positive charge modification to channels made with γ region I resulted in nearly 3-fold stimulation, in contrast to that observed to the furin cleaved channel in Figure 5. Those made with α region I was inhibited from the WT channel, in contrast to the no difference observed in Figure 5. These indicate marked differences in the interaction of these regions with the channel in the presence or absence of the tract between a.a. 205 and 221 in α region II (see text). N = 6–12. See Figure 3 legend for more details.