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. 2015 Sep 25;6(8):777–786. doi: 10.1080/21505594.2015.1091914

Figure 2.

Figure 2.

APEC and NMEC strains evade serum complement killing and survive inside bone marrow derived macrophages (BMDMs). Serum survival assays were performed with the APEC (AP) or NMEC (NM) strains as described in the Materials and Methods in the presence of normal and heat-inactivated serum, and percentage survival after 15 minutes exposure was compared to the corresponding values at 0 minutes for each individual strain. The decrease in survival with normal serum or increase in survival with heat-inactivated serum is statistically significant, *p < 0.05 by Student's t test. (A). Cell association (B) and invasion (C) patterns of the strains in BMDMs were also analyzed. The serum survival and cell association/assays were performed at least 3 times in triplicate, and the values are presented as percent means ± S.D considering E44 invasion as 100%. Increase or decrease in cell association/invasion was compared to E44, *p < 0.05 by Student's t test.