Table 3.

Survival of selected unique bacterial isolate from infant stool and breast milk in simulated bile, gastric juice and intestinal juice^a

	Simulated bile					Simulated gastric juice					Simulated intestinal juice
	OD 600					% survival					% survival
Isolate/ID	Source	5 hr	10 hr	24 hr	Overall	0	0.5 hr	1 hr	2 hr	Overall	0	1 hr	2 hr	4 hr	Overall
L. crispatus 330 L1	Fecal	0.2	0.6	1.2	++	100	92	0.8	0	+	100	70	40	10	+
L. fermentum 289 LT2	Isolate match	0.3	0.65	0.9	++	100	103	0	0	+	100	95	95	34	++
L. fermentum 297 R1	Fecal	0.2	0.4	0.6	+	100	187	25	0	+	100	78	106	86	++
L. gasseri 163 R1	Milk	0.2	0.3	0.3	+	100	109	93	27	++	100	55	27	2.3	+
L. gasseri 147 LT2	Fecal	0	0	0.1	—	100	101	40	30	++	100	32	1	0.3	—
L. gasseri 291 R3	Isolate match	0	0	0.3	—	100	99	81	17	++	100	7	2	0.2	—
L. gasseri 312 LT4	Isolate match	0.06	0.15	0.05	—	100	78	67	11	++	100	2	1	0.4	—
L. gasseri 163 L1	Milk	0.15	0.3	0.35	+	100	100	0	0	+	100	60	35	3	+
L. rhamnosus 141 L1	Fecal	0.1	0.6	1.1	+	100	0.2	0	0	—	100	80	74	63	+
L. rhamnosus 156 L1	Fecal	0.1	0.2	0.7	+	100	6	0	0	—	100	180	120	140	++
L. rhamnosus 153 LT1	Fecal	0.05	0.1	0.8	+	100	0.2	0	0	—	100	120	95	40	++
L. rhamnosus 321 L3	Fecal	0.06	0.1	0.65	+	100	75	0	0	+	100	105	70	100	++
E. casseliflavus 153 R3	Fecal	0.5	0.3	0.2	+	100	0.1	0.1	0	—	100	54	74	40	++
E. casseliflavus 153 R4	Fecal	0.09	0.35	0.3	+	100	0	0	0	—	100	135	90	85	++
E. faecalis 141 R4	Isolate match	0.3	0.5	0.8	++	100	1	0	0	—	100	117	100	97	++

^aSimulated bile: MRS + 0.3% oxgall; simulated gastric juice: NaCl + and pepsin (pH 2); simulated intestinal juice: NaCl + oxgall + pancreatin (pH 8)