Figure 1.
L. acidophilus-SlpA isolation by NaCl. L. acidophilus-SlpA was isolated and purified, as described previously,23 with substitution of LiCl with NaCl. (A) SDS-PAGE containing 2.5 μg of LiCl− and NaCl−isolated SlpA stained with Coomassie blue to visualize the purified protein. (B) Mass spectrometry data analyzed on the Scaffold27 platform showed 97 unique spectra with 55 unique peptides with the possibility of 2 proteins (C). The predicted protein gi|58336516 (SlpA) shows 54% coverage whereas gi|362076610 (SlpB) reveals only 18% of coverage (highlighted portion, D). The regions of SlpB matching the generated peptides are common between SlpA and SlpB (shown in the red box, D), and no single unique peptide from SlpB was identified.