Figure 1.
Induction of histamine release from HMC-1 cells by HP-NAP. HMC-1 cells were left unstimulated or stimulated with indicated concentrations of HP-NAP or 10 μM Ca2+ ionophore A23187 as a positive control at 37°C for 30 min. Histamine release from HMC-1 cells was determined by measuring the concentration of histamine in the cell-free supernatants as described in Materials and Methods. Data were represented as the mean ± SD of 4 independent experiments. *P < 0.05 as compared with unstimulated cells.