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. 2015 Sep 16;6(8):755–765. doi: 10.1080/21505594.2015.1043505

Figure 2.

Figure 2.

Induction of IL-6 release from HMC-1 cells by HP-NAP. (A) HMC-1 cells were left unstimulated or stimulated with 1 μM HP-NAP or 10 μg/mL E. coli LPS as a positive control at 37°C for the indicated time. IL-6 release from HMC-1 cells was determined by measuring the concentration of IL-6 in the cell-free supernatants as described in Materials and Methods. Data were represented as the mean ± SD of 3 independent experiments. *P < 0.05 as compared with unstimulated control cells at the same time point. (B) HMC-1 cells were left unstimulated or stimulated with 1 μM HP-NAP, 1 μM heat-inactivated (HI) HP-NAP, or 10 μg/mL E. coli LPS at 37°C for 16 h for measurement of IL-6 release as described in (A). Data were represented as the mean ± SD of 4 independent experiments. *P < 0.05 as compared with unstimulated control cells; #P < 0.05 as compared with HP-NAP-stimulated cells.