Figure 4.
Inhibition of HP-NAP-induced release of histamine and IL-6 from HMC-1 cells by the treatment with PTX. HMC-1 cells were pretreated with 100 ng/mL PTX at 37°C for 16 h and then stimulated with 1 μM HP-NAP at 37°C for 30 min or 16 h for measurement of the release of histamine or IL-6, respectively. Release of histamine and IL-6 from HMC-1 cells was determined as described in Figure 3. Data were represented as the mean ± SD of 6 independent experiments for histamine release and 5 independent experiments for IL-6 release. *P < 0.05 as compared with unstimulated cells in each group; #P < 0.05 as compared with HP-NAP-stimulated cells in the control group.