Figure 10.

Inhibition of HP-NAP-induced activation of ERK1/2, p38 MAPK, and Akt in HMC-1 cells by the treatment with U0126, SB203580, and LY294002. Serum-starved HMC-1 cells were not pretreated (control) or pretreated with 5 μM U0126, a MEK1/2 inhibitor, 10 μM SB203580, a p38 MAPK inhibitor, or 10 μM LY294002, a PI3K inhibitor, at 37°C for 1 h and then left unstimulated or stimulated with 1 μM HP-NAP at 37°C for 30 min. Cells were lysed and whole cell lysates were subjected to immunoblotting for phospho-ERK1/2, ERK, phospho-p38, p38, phospho-Akt, and Akt. The quantitative results were expressed in fold increase by defining the amounts of the phosphorylated proteins in cells without any treatment as 1 and represented as the mean ± SD of 3 independent experiments. *P < 0.05 as compared with unstimulated cells in each group; ^#P < 0.05 as compared with HP-NAP-stimulated control cells.