Figure 2.
Dengue virus binding to platelets involves DC-SIGN and HSP. Platelets (5 × 105/mL) were incubated for 30 minutes at (A) 4°C, (B) 25°C, or (C) 37°C with PBS, purified Fc (TruStain FcX), nonimmune isotype-matched control IgG, anti-DC-SIGN, LMW heparin, chondroitin sulfate, or a combination of LMWH and anti-DC-SIGN (each at 50 μg/mL). Platelets were then incubated with purified DENV2 (4 × 107 particles/mL) for 1 hour. Platelets were washed twice with PBS, and RNA was isolated for quantification of bound virus by qRT-PCR. (n = 3, ±SD.)