Figure 5.

Dengue virus protein is translated by platelets. (A) Western blot analysis was used to detect DENV-encoded NS1 from aliquots of inoculated platelets cultured as in Figure 4. The ∼45-kDa band was quantified by densitometry post- (filled squares) and preinoculation (open squares). The fit of data is arbitrary. (B) Representative blots showing NS1 antigen generation, invariant β-actin as a control for loading and specificity of gene replication, and the lack of antigenic detection in the absence of DENV2.