Skip to main content
. 2016 Jan 13;7(4):3709–3725. doi: 10.18632/oncotarget.6915

Figure 3. IFNα/BV6-induced cell death depends on RIP1.

Figure 3

Figure 3

Cells were transiently transfected with 5 nM siRNA targeting RIP1 (siRIP1-1, siRIP1-2) or control siRNA. A. Cells were treated with IFNα (A172: 5 ng/ml, HT-29: 10 ng/ml) and 1 μM BV6. Protein expression of RIP1 was analyzed by Western blotting, GAPDH was used as loading control. B. Cells were treated for 72 hours with IFNα (A172: 5 ng/ml, HT-29: 10 ng/ml) and/or 1 μM BV6. Cell death was determined by analysis of DNA fragmentation of PI-stained nuclei using flow cytometry. Mean + SD of three independent experiments performed in duplicate are shown; *P < 0.05; **P < 0.01. C. Cell viability was determined by MTT assay. Data are shown as percentage of untreated control cells with mean + SD of three independent experiments performed in triplicate; *P < 0.05; **P < 0.01. D. Cells were treated for 24 hours with IFNα (A172: 5 ng/ml, HT-29: 10 ng/ml) and 1 μM BV6. Cleavage of caspase-8 and 3 was assessed by Western blotting, GAPDH was used as loading control.