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. 2015 Dec 24;7(4):3777–3790. doi: 10.18632/oncotarget.6756

Figure 5. Blocking miR-21 largely abolishes the effect of LIF on inducing EMT.

Figure 5

(A and B) MCF7 and T47D cells with and without stable ectopic expression of LIF were transfected with anti-miR-21 or control RNA oligos. The mRNA (A) and protein (B) levels of EMT markers were determined by real-time PCR and Western blot assays, respectively. The mRNA levels of these genes were normalized to β-actin. Data are presented as mean ± s.d. (n = 3). (C) Blocking miR-21 largely abolished the promoting effect of LIF on migration ability in MCF7 and T47D cells. MCF7 and T47D cells with and without stable ectopic LIF expression were transfected with anti-miR-21 or control RNA oligos. The migration ability of cells was determined by trans-well assays. Left panels: representative images; right panels: quantification of average number of migrated cells/field. *p < 0.05; student t-test.