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. 2015 Dec 24;7(4):3777–3790. doi: 10.18632/oncotarget.6756

Figure 7. STAT3 mediates the promoting effect of LIF on EMT in MCF7 and T47D cells.

Figure 7

(A and B) Blocking STAT3 function by Stattic largely abolished the effect of LIF on the expression of EMT markers. MCF7 and T47D cells with ectopic stable LIF expression were treated with Stattic (2 μM) for 24 h. The expression levels of EMT markers were determined at both mRNA (A) and protein (B) levels by real-time PCR and Western blot assays, respectively. (C and D) Knockdown of endogenous STAT3 by siRNA largely abolished the effect of LIF on the expression of EMT markers. MCF7 and T47D cells with ectopic stable LIF expression were transfected with siRNA targeting STAT3 or control siRNA. The expression levels of EMT markers were determined at both mRNA (C) and protein (D) levels by real-time PCR and Western blot assays, respectively. The levels of total STAT3 and phosphorylated STAT3 at Tyrosine 705 (p-STAT3) were determined by Western blot assays (B and D). For A and C, data are presented as mean ± s.d. (n = 3). *p < 0.05; student t-test.