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. 2015 Nov 10;7(4):3791–3805. doi: 10.18632/oncotarget.6302

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Copyright: © 2016 Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Expression of CISD2 was frequently upregulated in 409 gastric tumor tissues (Tumor) compared with 37 adjacent normal gastric tissue samples (Adjacent) in the TCGA profile. B. CISD2 expression was markedly increased in 37 paired gastric tumor tissues (Tumor) and their adjacent normal tissues (Adjacent) in the TCGA profile. C. Real-time PCR analysis of CISD2 expression in NGEC and gastric cancer cell lines. D. Western blotting of CISD2 expression in NGEC and gastric cancer cell lines. E. CISD2 protein expression level in five paired gastric tumor tissues (T) and their adjacent normal tissues (ANT). F. CISD2 mRNA expression level in five paired gastric tumor tissues (T) and their adjacent normal tissues (ANT). The average CISD2 mRNA expression was normalized to the expression of GDPDH. α-Tubulin was detected as a loading control in the Western blot. Three independent experiments were conducted for each assay.