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. 2015 Dec 16;7(4):3819–3831. doi: 10.18632/oncotarget.6625

Figure 2. PA induces G0/G1 phase arrest of prostate cancer cells.

Figure 2

A. PC3, DU145, and NB26 were treated with 20-40μM PA for 24h, fixed with 1% paraformaldehyde and cell cycle distribution was evaluated after propidium iodide staining. Cells were analyzed by flow cytometry. Percentage of cell population in G0/G1-phase of the cell cycle is shown in the table. Experiments were performed in triplicate and mean ± SD is shown. B, C & D. Effect of PA treatment on cell cycle regulatory proteins: Whole cell lysates of PC3, DU145 and NB26 cells with/without PA (20–40μM:24h) were subjected to SDS-polyacrylamide gel electrophoresis. Equal loading was confirmed by reprobing with GAPDH. The immunoblots shown are representative of three independent experiments with similar results.