Skip to main content
. 2015 Dec 26;7(4):3984–3992. doi: 10.18632/oncotarget.6766

Figure 2. Target inhibition by single and combined, low-concentration siRNA treatment.

Figure 2

Human and murine PDAC cell lines were transfected with 12 nM siRNAs per target gene for 72 h. For single target inhibition the siRNA was filled up with nonsense siRNA to 72 nM. For simultaneous gene silencing (SGS6) six siRNAs, each with a concentration of 12 nM, were used. (A) On transcriptional level knockdowns were confirmed by qRT-PCR relative to their negative control (n ≥ 2; *p ≤ 0,05, **p ≤ 0,01, ***p ≤ 0,001). (B) On protein level silencing was always confirmed by Western blots relative to Tubulin as housekeeping protein.