Figure 5. Treatment of subcutaneous TB32047-allografts with the SGS6 therapy.

Subcutaneous, bilateral TB32047 tumors of NMRI^nu/nu mice were treated daily with 10 μg of in vivo jet-PEI complexed SGS6 siRNA for 8 d (11–14 tumors per group). (A) Tumor volumes were measured during therapy and (B) their weight was analyzed after preparation at day 9. Means ± standard errors are shown and an unpaired Student's t-test was used to compare the differences between the negative control (NC) and the SGS6 treated tumors. (C) Exemplary photos are depicted. (D) Gene silencing in SGS6 treated tumors was confirmed by qRT-PCR related to the tumors treated with nonsense siRNA (*p ≤ 0,05, **p ≤ 0,01, ***p ≤ 0,001).