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. 2015 Dec 26;7(4):4142–4154. doi: 10.18632/oncotarget.6769

Figure 2. Treatment of HER2-amplified breast cancer cells with RY10-4 induces Notch signaling.

Figure 2

(A) Measurement of CSL luciferase reporter activity in MDA-MB-231, MCF-7, SKBR3, and BT474 cells after 6 h of RY10-4 treatment. (B–G) qPCR analysis of Notch1, Notch4, Dll4, Jagged1, Hes1, and Hey1 gene expression in MDA-MB-231, MCF-7, and SKBR3 cells in the absence and presence of RY10-4 over a 6-h time course. Expression of Notch signaling genes was normalized to GAPDH levels. The data represent mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, versus vehicle control.