Figure 7. DAPT and RY10-4 inhibit the growth of SKBR3 xenografts.
BALB/c nude mice were challenged subcutaneously with 1 × 106 SKBR3 cells for seven days, followed by administration with vehicle, DAPT (100 mg/kg, QD), RY10-4 (200 mg/kg, QOD), or DAPT + RY10-4 (n = 5 mice per group) for another twelve days. The mice were euthanized, and the tumor tissue was dissected and weighed. (A) A schematic diagram depicting the experimental protocol used for tumor xenografts. (B) Representative pictures of isolated tumors. (C) Measurement of tumor volume. (D) Measurement of tumor mass from different groups of mice. Data are expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, versus vehicle control group. (E) Tumors were cut into sections, and immunohistochemistry staining was performed. Representative images showing staining for Dll4, Hes1, and cleaved caspase-3 from the control, DAPT-treated, RY10-4-treated, and combination-treated groups are shown at ×400 magnification.