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. 2015 Dec 10;7(4):4195–4209. doi: 10.18632/oncotarget.6549

Figure 4. Knockdown of Sp1 and MeCP2 regulate TLR4 expression through modification of DNA and histone methylation.

Figure 4

A. qMSP analysis of CpG sites in TLR4 promoter in gastric cancer cells transfected with control short interfering RNA (siRNA) and siRNA targeting MeCP2 (left) or Sp1 (right) and treated with lipopolysaccharide (LPS) and 5-aza-dC. # p < 0.01. B. Western analysis of the indicated proteins in nuclear fraction of cells described in (A) to monitor modifications in methylation of histones. C. qPCR analysis of TLR4 expression level, normalized to that of β-actin, in cells described in (A). *p < 0.05, #p < 0.01.