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. 2015 Dec 7;7(4):4252–4264. doi: 10.18632/oncotarget.6483

Figure 1. Upregulation of FLOT1 correlates with lymph node metastasis in human NPC.

Figure 1

(A) Western blotting analyses of FLOT1 expression in two non-cancerous nasopharyngeal tissues and tumor tissues from patients with NPC with (LN+, n = 14) and without (LN−, n = 8) lymph node metastasis. GAPDH was used as a loading control. (B) Quantification of western blotting analysis of FLOT1 expression; the average ratio of FLOT1/GAPDH in the two non-cancerous nasopharyngeal tissues was considered as 1.0. Error bars represent mean ± SEM. P < 0.001, Student's two-tailed t-test. (C) Western blotting analyses of FLOT1 expression in 6 cultured NPC cell lines compared with normal nasopharyngeal epithelial cells (NPECs). (D) Representative photomicrographs (left) and the percentages (right) of specimens with high and low levels of FLOT1 expression in tumor tissues from patients with NPC with (LN+, n = 112) or without (LN−, n = 57) lymph node metastasis. Scale bars: 50 μm. Right panel: The Chi-square test was used for the correlation analysis. (E) Kaplan–Meier overall survival and disease-free survival curves for patients with NPC stratified by low and high levels of FLOT1 expression (n = 169; log-rank test). (F) Correlation of FLOT1 expression with overall survival in the subgroup of patients with lymph node metastasis (n = 112; log-rank test).