Skip to main content
. 2015 Dec 7;7(4):4252–4264. doi: 10.18632/oncotarget.6483

Figure 3. FLOT1 promotes NPC cell invasion and lymph node metastasis in vivo.

Figure 3

(A) An inguinal lymph node metastasis model was established by inoculating the foot pads of nude mice with CNE2 cells. (B) Upper panel: H & E staining revealed the tumors formed by FLOT1-overexpressing CNE2 cells (c, d, e and f) exhibited a more aggressive phenotype than the vector control cells (a and b), with the FLOT1-overexpressing tumor cells invading into the muscles (c and d), and towards the skin (e), and even into lymphatic vessels (f; asterisk represents tumor cells; arrowheads represent lymphatic vessel). T: tumor; S: skin; M: muscle; LV: lymphatic vessel. Lower panel: Quantification of the amount of peritumoral lymphatic invasion in histologic sections of the tumors formed by FLOT1-overexpressing and vector control CNE2 cells. (C) Representative micrographs (left) and volumes of the inguinal lymph nodes (right). (D) Representative micrographs of the inguinal lymph nodes immunostained with an anti-pan-cytokeratin antibody. Scale bars: 50 μm. (E) Ratios of metastatic inguinal lymph nodes to the total number of inguinal lymph nodes dissected in mice inoculated with the indicated cells, the Chi-square test was used for statistical analysis. Error bars represent mean ± SD; *P < 0.05.