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. 2015 Dec 7;7(4):4252–4264. doi: 10.18632/oncotarget.6483

Figure 4. Downregulation of FLOT1 suppresses the aggressiveness of NPC cells.

Figure 4

(A and B) Wound healing assay (A) and transwell matrix invasion assay (B) showing that silencing of FLOT1 reduced NPC cell migration and invasion. (C) Representative micrographs (upper) and quantification analysis (lower) of the indicated cells cultured in the 3-D spheroid invasion assay. Scale bars: 20 μm. (D) Representative micrographs (left panel) and the volumes of inguinal lymph nodes in mice inoculated with the indicated cells in the inguinal lymph node metastasis model (right panel). (E) Representative micrographs of inguinal lymph nodes immunostained with an anti-pan-cytokeratin antibody. Scale bars: 50 μm. (F) Ratios of metastatic inguinal lymph nodes to the total number of inguinal lymph nodes dissected in mice inoculated with the indicated cells, the Chi-square test was used for statistical analysis. Error bars represent mean ± SD from three independent experiments; *P < 0.05.