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HCT116 cells were pretreated with 50 nM chloroquine for 6 h and then exposed to 800 μM niacin for 12 h and 100 ng/ml TRAIL for 2 h. A, B. Western blot analysis of p62 and LC3. β-actin was used as a loading control. C. Representative images of LC3 and p62 protein expression in HCT116 cells.
