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. 2015 Dec 21;7(4):4442–4453. doi: 10.18632/oncotarget.6710

Figure 5. p130Cas expression influences ErbB2 ubiquitination.

Figure 5

(A) Left panel: Cell extracts from control (Ctr), p130Cas silenced and p130Cas overexpressing BT474 cells were immunoprecipitated with ErbB2 antibodies, followed by immunoblotting against Ubiquitin and ErbB2 antibodies. Right panel: Histograms show the levels of ubiquitin, normalized to ErbB2. Bars represent the means ± SEM of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001). (B) Cell extracts as in A were probed with antibodies to p130Cas, ErbB2 and normalized with Vinculin. (C) Cell extracts from control (Ctr), p130Cas silenced and p130Cas overexpressing BT474 cells were immunoprecipitated with ErbB2 antibodies and blotted with Cbl, CHIP and ErbB2 antibodies.