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. 2015 Dec 21;7(4):4442–4453. doi: 10.18632/oncotarget.6710

Figure 6. p130Cas overexpression promotes resistance to trastuzumab.

Figure 6

(A) Quantification of p130Cas mRNA by qRT PCR in wild type SKBR3 (SKBr3), in trastuzumab resistant SKBR3 (SKBr3R), in BT474 wild type (BT474) and in trastuzumab resistant BT474 cells (BT474R). The 18S housekeeping gene was used as an internal control for data normalization. (B) Cell extracts from trastuzumab sensitive and resistant SKBR3 and BT474 cells were probed with p130Cas antibodies and normalized with Actin. (C) Cell extracts from p130Cas overexpressing BT474 cells and relative control cells untreated or treated with 100 nM Rapamycin (R), 10 μM/ml trastuzumab (T) alone or in combination (R + T) were blotted with antibodies against ErbB2, p130Cas, phospho-S6, LC3 and GAPDH as loading control. (D) Representative images of p130Cas expression in primary tumor at the time of diagnosis (left panel) and in relapsing trastuzumab resistant tumor (right panel) of patient n°2 (see Table 1).