Figure 2. Binding of the two most abundant TIM3 aptamers to the mouse recombinant protein TIM3.

A. Binding of TIM3-Apt1 TIM3Apt2 performed by filter-binding assay described in methods to the chimeric recombinant protein mTIM3-Fc; a randomized Apt control library was used as a negative binding control. B. Binding of TIM3-Apt1, TIM3-Apt2 and Apt-control to IgG1 protein.