Figure 2. Alterations in nuclear texture in vitro and in vivo after treatment with platinum drugs or radiotherapy.

A. Changes in nuclear texture in PEO1 cells after irradiation or cisplatin treatment. PEO1 cells were grown on coverslips and treated with ionising radiation (6Gy) or cisplatin (6uM) for 24h, and nuclei were stained with DAPI for visualisation using a fluorescence microscope. At least 100 nuclei were included in each experiment. Nuclear texture was analysed by measuring five texture parameters (angular second moment, correlation, entropy, inverse different moment, and contrast) using Image J software. Data are presented as the average change (%) in the treated group for each parameter over the control group. B. Representative images from H&E-stained OV1002 ovarian tumor samples either untreated or after carboplatin (50 mg/kg) treatment in vivo. H&E stained images were acquired under 40x magnification. C. Nuclear texture parameter analysis in xenografts with and without carboplatin treatment on Day 2. Data for each spot represents the average value of each single sample with the number of nuclei analysed per sample ranging from 16 to 213 (average 103). Mann-Whitney U test (2-tailed); *P<0.05, **P<0.01 D. Changes in chromatin patterns in PEO1 cells after cisplatin (6uM) or radiation (6Gy) treatment for 24h, and in OV1002 xenografts in vivo after carboplatin treatment, measured by parameters describing nuclear texture using Image J. The (+) and (−) represent positive and negative correlations with each type of chromatin pattern, respectively, and the arrows indicate the direction of change for each pattern.