Figure 6. The effect of siHDAC2 knockdown on expression of other HDAC family members, HP1s, and DNA damage response proteins by western blotting in PEO1 cells alone (A) and in the presence / absence of cisplatin (B).

Protein was lysed after HDAC2 was knocked down after 72 h using reverse transfection. Non-transfection (control), mock, and siRNA negative control were used as controls. Membranes were probed with the indicated antibodies, and tubulin was used as a loading control. Experiments were performed at least three times acquiring similar results. Blots from one representative experiment are shown. In (B), cells were treated with cisplatin (6μM) after 72h incubation with mock (lane 1), siRNA negative control (lane 2), or HDAC2 siRNA duplexes (10pmol in 6mL, lane 3). Protein lysates were collected at 6h, 24h, 48h, and 72h after cisplatin treatment and analysed by western blotting of DNA damage response proteins; tubulin was used as loading control. Experiments were performed at least three time acquiring similar results. Blots from one representative experiment are shown.