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. 2015 Dec 15;7(4):4724–4734. doi: 10.18632/oncotarget.6619

Figure 3. GNB contains a shell composed of drumstick-shaped subunits and inner structure.

Figure 3

A. Immunofluorescent staining images of purified GNBs (asterisks) and the subunits of GNB shells (arrowheads) from KCL-22M leukemia cells. B. Structure image of an intact GNB with a shell composed with several subunits. C. Structure image of a single subunit of the shell contains a head, a stem and a tail. D–F. Profile analyses of p-eIF4E protein (D), SUMO1-modified proteins (E), and SUMO2/3-modified proteins (F) of total cell lysates and purified GNB lysates using Western blotting with antibodies against p-eIF4E, SUMO1 or SUMO2/3. G. Two-color confocal images of p-eIF4E (green) and SUMO1(red) in GNB by immunofluorescence staining. GNBs were incubated with antibodies against p-eIF4E and SUMO1 followed by a secondary antibody labeled with FITC (p-eIF4E, green color) and Alexa Fluor 568(SUMO1, red color). A colocalization of p-eIF4E and SUMO1 in the GNB was evident (merge, yellow). H. SUMOylation inhibition decreased GNBs in KG-1 leukemia cells. Leukemia cells were treated with GA at 200 μM for different time points and then collected for analyses of GNBs using immunofluorescence staining. Cell nuclei (blue) were stained using DAPI.