Table 1. Specifications of primary antibodies used in the TMA.
Marker | Clone | Brand | Dilution | pH antigen retrieval | Time of antigen retrieval | Positive Control |
---|---|---|---|---|---|---|
CD10 | 56C6 mouse | Dako | 1:2 | Tris-EDTA pH 9.0 | 20 min 97°C | Palatine tonsil |
BCL6 | LN22 mouse | Novocastra | 1:100 | Citrate pH 6.1 |
20 min 97°C | Palatine tonsil |
MUM1 | MUM1p mouse | Novocastra | 1:1200 | Citrate pH 6.1 |
20 min 97°C | Palatine tonsil |
CD68 | KP1 mouse | Dako | 1:6000 | Citrate pH 6.1 |
20 min 97°C | Palatine tonsil |
SPARC | H-90 | Santa Cruz Biotechnology | 1:500 | Citrate pH 6.1 |
20 min 97°C | Kidney |
CD34 | QBEnd10 mouse | Dako | 1:800 | Citrate pH 6.1 |
20 min 97°C | Prostate |