Figure 5. Effect of pig7 on the expression and cytosolic release of cathepsins in leukemia cells, when comparing isolated lysosomes and non-lysosome-containing lysate.

Overexpression of pig7 resulted in: (i) active cathepsin B, D and L release from lysosomes, (ii) increased precursors pro-cathepsin in B, D, and L were cleaved to their active forms, but fewer precursors were released into the cytosol, and (iii) the combination of VP16 further enhanced the above effects.