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. 2015 Dec 17;7(4):4939–4948. doi: 10.18632/oncotarget.6643

Figure 3. CCK stimulates CTSB secretion by the adipocytes.

Figure 3

A. Protein secretion profiles of 3T3-L1-derived adipocytes before or after being co-cultured with TRAMP-C1 cells was determined by mass spectrometry. Proteins upregulated under the co-culture condition were listed in Table A. B&C. ELISA and RT-PCR were performed to validate the induction of CTSB expression in adipocytes after co-culture with TRAMP-C1 cells. (D&E) Secretion of CTSB in adipocytes is regulated by CCK. D. 3T3-L1-derived adipocytes were treated with CCK recombinant protein (0.2μg/ml) for 4 hours and the level of CTSB in the conditioned medium was analysed by ELISA. E. Addition of YM022 (1 and 5 μM) completely abolished the effects of CCK on CTSB induction. Each experiment was repeated at least three times, and the results are presented as the mean ± SD. (p values: * < 0.05, ** < 0.005).