Skip to main content
. 2015 Dec 18;7(4):5007–5022. doi: 10.18632/oncotarget.6652

Figure 3. The PRDM16 gene is a direct target of miR-101.

Figure 3

A. The PRDM16 gene was predicted to be a target of miR-101 using the online software program TargetScan 5.1. B. The miR-101 targeting site in PRDM16 (3′-UTR) is shown. Wild-type and mutated PRDM16 3′-UTRs are shown. C. miR-101 regulates the expression of PRDM16 3′-UTR reporter constructs. The luciferase reporter assays were performed 48 h after transfection with the indicated pMIR-REPORT plasmid and a Renilla transfection control plasmid, which were co-transfected with miR-101 or a scrambled control. The data shown are means ± S.D.s of six replicates and are representative of three independent experiments. An independent samples t-test was used. *P<0.05. D. miR-101 inhibits the expression of PRDM16 mRNA. Real-time PCR analysis was performed 48 h after transfection with miR-101 and a scrambled control. An independent samples t-test was used. *P<0.05. E. miR-101 regulates the expression of the PRDM16 protein in four astrocytoma cells. Western blot analysis was performed 72 h after transfection with miR-101 and a scrambled control. GAPDH was used as an internal control.