Skip to main content
. 2015 Dec 18;7(4):5007–5022. doi: 10.18632/oncotarget.6652

Figure 5. miR-101 induces cellular apoptosis via a mitochondrial pathway.

Figure 5

A. Mitochondrial membrane potentials were monitored by using JC-1 staining. U251 cells were transfected with miR-101 or a scrambled control. Red fluorescence indicates normal U251 cells, and green indicates cells with mitochondrial dysfunction. B, C. and D. Mitochondrial ATP levels, ADP/ATP ratios and ROS production were detected 48 h after transfection with miR-101 or a scrambled control. E. Flow cytometry analysis was performed 48 h after U251 cells transfection with miR-101 or a scrambled control. F. DAPI staining analysis was performed 48 h after U251 cells were transfected with miR-101 or a scrambled control. G. Western blot analysis of Bax, Bcl-2, caspase-9, c-PARP and Smac expression levels after transfection with miR-101 or a scrambled control. GAPDH and VDAC were used as loading controls for cytoplasmic protein and mitochondrial protein measurements, respectively.