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. 2016 Apr 5;24(4):528–536. doi: 10.1016/j.str.2016.02.008

Figure 2.

Figure 2

BK Polyomavirus VLP Production in HEK293TT Cells

BK Polyomavirus VLPs produced in HEK293TT cells can package plasmid DNA and can be used as a gene delivery vector.

(A) Western blot for VP1 expression in each fraction from the iodixonal gradient. 20 μl of the gradient solution was loaded and VP1 levels were detected using a VP1-specific antibody.

(B) Purified VLPs were used to transduce HEK293TT, and the presence of EGFP was analyzed by light microscopy. The image is representative and approximately 65% of the cells were transduced.

(C) Negative-stain micrograph of purified VLPs.

(D) Cryo-electron micrograph of purified VLPs suspended in vitreous ice.

Scale bars, 100 nm.