Table 1.
Crystallization and Structure Determination for hN1 EGF4-7
| Space Group | P21 | C2 |
|---|---|---|
| Cell | ||
| a, b, c (Å) | 40.94, 86.83, 53.45 | 142.26, 21.15, 83.56 |
| α, β, γ (°) | 90, 107, 90 | 90, 116.27, 90 |
| Wavelength (Å) | 1.74626 | 1.74626 |
| Resolution (Å) | 43.42–2.46 (2.69–2.46) | 64.73–2.92 (3.26–2.92) |
| Rmerge (%) | 3.0 (38.8) | 3.1 (36.8) |
| I/σI | 23.6 (2.3) | 17.4 (2.1) |
| Completeness (%) | 89.9 (93.8) | 95.0 (98.0) |
| Redundancy | 3.3 | 3 |
| Number of reflections | 39,951 | 15,128 |
| Rwork/Rfree (%) | 25.9/26.3 | 21.4/23.7 |
| Number of atoms | ||
| Protein | 4,154 | 2,099 |
| Ligand/ion | 29 | 8 |
| Water | 18 | 6 |
| B factors | ||
| Protein | 69.2 | 34.4 |
| Ligand/ion | 66.0 | 40.5 |
| Water | 56.0 | 17.3 |
| RMS deviation | ||
| Bond length (Å) | 0.035 | 0.01 |
| Bond angles (°) | 1.21 | 1.23 |
| Residues in allowed regions of Ramachandran plot (%) | 100 | 100 |
| Residues in favored regions of Ramachandran plot (%) | 97.3 | 95.4 |