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. Author manuscript; available in PMC: 2017 Mar 27.
Published in final edited form as: J Mol Biol. 2016 Feb 22;428(6):1053–1067. doi: 10.1016/j.jmb.2016.02.017

Figure 3. A monomer of Pif1 has helicase activity under multiple turnover conditions.

Figure 3

FRET-based unwinding experiments monitoring the increase in Cy3 fluorescence using 20 nM of the indicated substrates in complex with 15 nM Pif1238–859. The reactions were started by addition of either 0.5 mM ATP (blue) or 0.5 mM ATP and a trap to prevent re-annealing of the unwound dsDNA in 3.5-fold excess (3.5x) relative to the DNA (r.a. TRAP, red).