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. Author manuscript; available in PMC: 2017 Mar 27.
Published in final edited form as: J Mol Biol. 2016 Feb 22;428(6):1053–1067. doi: 10.1016/j.jmb.2016.02.017

Figure 7. In excess Pif1 unwinding is limited by the Pif1-dependent annealing activity. a).

Figure 7

FRET-based unwinding experiments using 20 nM of the indicated substrates and 200 nM Pif1238–859, starting the reactions by addition of either 0.5 mM ATP (blue) or 0.5 mM ATP and 3.5x r.a. TRAP (red). b) Same experiments as in a) comparing the unwinding activity of Pif1238–859 and Pif1238–780 using substrates that do not contain a 3′-tail.