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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Biomol NMR. 2016 Feb 12;64(3):223–237. doi: 10.1007/s10858-016-0023-3

Figure 3.

Figure 3

2D 13C-13C dipolar INADEQUATE spectra of D44A-M2TM in DMPC bilayers. (a) Spectrum measured at 283 K on a 400 MHz spectrometer. (b) Spectrum measured at 203 K on a 400 MHz spectrometer. (c) Spectrum measured at 120 K using DNP on a 400 MHz spectrometer. (d) Spectrum measured at 116 K using DNP on a 600 MHz spectrometer. The sample for (a–b) does not contain cryoprotectant or radicals and used protonated DMPC. The sample for (c–d) used glycerol-protected d54-DMPC and 10 mM AMUPol (sample 1). The lowest contour level for all 2D spectra is at 15% of the highest intensity of each spectrum, and 24 contour levels are shown. Representative 1D cross sections are extracted to compare 13C linewidths. The non-methyl 13C signals broadened by 0.1–0.4 ppm from the 283 K 400 MHz spectrum to the 116 K 600 MHz DNP spectrum.