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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Biomol NMR. 2016 Feb 12;64(3):223–237. doi: 10.1007/s10858-016-0023-3

Figure 4.

Figure 4

2D 15N-13C correlation spectra of membrane-bound M2TM under different conditions to examine 15N spectral resolution. (a) Spectrum of DMPC-bound M2TM at 283 K without DNP on a 400 MHz spectrometer. (b) Spectrum of DLPE-bound M2TM at 120 K measured on a 400 MHz DNP spectrometer (sample 9). (c) Spectrum of d54-DMPC bound M2TM at 116 K measured on a 600 MHz DNP spectrometer (sample 1). (d) Spectrum of d54-DMPC bound M2TM at 165 K measured on a 600 MHz DNP spectrometer. The samples for (b–d) are cryoprotected with glycerol containing 10 mM AMUPol. All spectra were plotted with the lowest contour at 20% of the highest peak in each spectrum and 16 contour levels are plotted.