Table 1.

Measured DNP enhancement factors ε_C,CP at 400 MHz.

Samples	Varied parameters	Sample number	Cryoprotectant and biradical	εC,CP ≡ IMW on/IMW off
M2TM in DMPC	d54-DMPC	1	d8-glycerol, 10 mM AMUPol	80 (C′), 100 (Cα), 69 (glycerol) 60 (C′), 56 (Cα), 56 (lipid CH2), 78 (glycerol)
	1H-DMPC	2
M2TM in 1H-DMPC	d8-glycerol	2	10 mM AMUPol	60 (C′), 56 (Cα), 56 (lipid CH2), 78 (glycerol) 39 (C′), 39 (Cα), 38 (lipid CH2), 63 (DMSO)
	d6-DMSO	3
M2TM in 1H-DMPC	Titration	3	d6-DMSO, 10 mM AMUPol	39 (C′), 39 (Cα), 63 (DMSO) 22 (C′), 27 (Cα), 50 (DMSO)
	Centrifugation	4
ROCKER in 1H-DMPC	Titration	5	d6-DMSO, 10 mM AMUPol	26 (C′), 40 (Cα), 45 (lipid CH2), 85 (DMSO) 19 (C′), 14 (Cα), 13 (lipid CH2), 22 (DMSO)
	Centrifugation	6
VM+ membrane	10 mM AMUPol	7	d8-glycerol	42 (lipid CH2), 60 (glycerol) 10 (lipid CH2), 20 (glycerol)
	10 mM TOTAPOL	8
M2TM	1H-DMPC	2	d8-glycerol, 10 mM AMUPol	60 (C′), 56 (Cα), 56 (lipid CH2), 78 (glycerol)
	1H-DLPE	9		58 (C′), 75 (Cα), 64 (lipid CH2), 78 (glycerol)