FIG. 1.
Procedure for investigating intracellular calcium signalling of cells using different stimulation mechanisms. (a) Discontinuous dielectrophoresis process, image adapted from our recently published work entailing the complete discontinuous dielectrophoresis procedure.39 (b) Stimulation of immobilised cells using different mechanisms: (i) Investigating chemical induced calcium signalling, achieved by applying agonists such as GSK1016790A, at a low flow rate of 2.5 μl/min. (ii) Investigating shear stress induced calcium signalling of cells, achieved through varying the flow rate applied to the microfluidic platform (such as 120 μl/min). (iii) Investigating concurrent chemical and shear stress induced calcium signalling of cells. Scale Bar: 50 μm.