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. 2016 Apr 4;10(2):024117. doi: 10.1063/1.4945309

FIG. 2.

FIG. 2.

Comparing the influence of LEC and HBS suspensions, in regard to the intracellular calcium signalling of HEK-293-TRPV4 cells. (a) and (b) Normalised intensity profiles at different concentrations of GSK1016790A (3.125 nM and 12.5 nM, respectively), conducted for HEK-293-TRPV4 cells suspended in (i) LEC, (ii) HEPES, and (iii) NT HEK-293 cells suspended in HEPES (Negative control) (see supplementary material S3 for experimental fluorescent images).46 (c) Comparing the response characteristics: (i) cellular and (ii) peak response times, and (iii) pharmacological efficacy of cells suspended in different buffers. Data are presented as the mean ± SEM, ***P < 0.001, and ****P < 0.0001.